Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and

產(chǎn)品型號：Z02691

更新時(shí)間：2025-11-06

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產(chǎn)品介紹

產(chǎn)品簡介

Species

Human

Protein Construction

Expressed with an N-terminal Met.

EGF (Asn971-Arg1023)
Accession # P01133

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 1 EU/μg of protein by gel clotting method

Biological Activity

ED50 < 0.6 ng/ml, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of > 1.7 × 106 U/mg.

Expression System

E. coli

Apparent Molecular Weight

~6.2 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against 10 mM phosphate buffer, pH 7.0, 200 mM NaCl buffer.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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