Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. M

產(chǎn)品型號(hào)：Z02774

更新時(shí)間：2025-11-06

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產(chǎn)品介紹

產(chǎn)品簡(jiǎn)介

Species

Mouse

Protein Construction

Expressed with an N-terminal Met.

TNF-α (Leu80-Leu235)
Accession # P06804

Purity

> 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC

Endotoxin Level

< 1 EU/μg of protein by LAL method

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D.

Expression System

E. coli

Theoretical Molecular Weight

17.4 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. Avoid repeated freeze-thaw cycles.

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