Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a fo
> 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC
Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Biological Activity
ED50 < 0.1 ng/ml, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1.0 × 107 U/mg.
Expression System
CHO
Apparent Molecular Weight
17~30 kDa, on SDS-PAGE under non-reducing conditions.
Formulation
Lyophilized after extensive dialysis against PBS.
Reconstitution
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.
Storage & Stability
Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.