MonoRab™ Anti-DYKDDDDK Affinity Resin：GenScript’s MonoRab?? Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can b

產(chǎn)品型號(hào)：L00766

更新時(shí)間：2025-11-06

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產(chǎn)品介紹

產(chǎn)品概述

Product Description

DYKDDDDK or FLAG® tag is an octapeptide that can be added to a protein using recombinant DNA technology. The tag sequence can be fused to the coding sequence of a target protein to facilitate detection and purification. GenScript’s MonoRab™ Anti-DYKDDDDK Affinity Resin (Cat. No. L00766) is designed for optimal purification of DYKDDDDK-tagged proteins. Based on our new rabbit monoclonal antibody technology (MonoRab™), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid. The binding capacity of MonoRab™ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5. Table 1 lists the main characteristics of the MonoRab™ Anti-DYKDDDDK Affinity Resin.

產(chǎn)品屬性

Product Content	50% settled resin in TBS with 50% glycerol and 0.02% sodium azide
Matrix	4% cross-linked agarose
Average Bead size	90 μm
Ligand	MonoRab™ monoclonal antibody against DYKDDDDK tag
Binding Capacity	Approximately 1.5 mg DYKDDDDK-tagged protein (Size: 50KDa) per ml settled resin
Elution Method	Two methods can be implied for elution: 1. Alkaline, Elution under native conditions by competition with peptide 2. Elution with SDS-PAGE loading buffer*.
Resin Reuse	The resin can be recycled for at least 4 times. If maintained as per manufacturer’s instructions, the resin can be reused 10 times with minimum loss of binding capability
Reagents Compatibility	Compatible with commonly used reagents at certain concentrations
Storage & Stability	Store at -20 °C for 12 months.