IL-4, Human：is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival

Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.

產(chǎn)品簡(jiǎn)介SpeciesHumanProtein ConstructionIL-4 (His25-Ser153)Accession # P05112Purity> 95% as analyzed by SDS-PAGE> 95% as analyzed by HPLCEndotoxin Level< 0.2 EU/μg of protein by gel clotting methodBiological ActivityED50 < 0.25 ng/ml, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of > 4.0 × 106 U/mgExpression SystemCHOApparent Molecular Weight~15 kDa, on SDS-PAGE under non-reducing conditions.FormulationLyophilized after extensive dialysis against PBS.ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.Storage & StabilityUpon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.